16s rdnaqizhongleishao，hanliangda，fenzidaxiaoshizhong，cunzaiyugezhongyuanheshengwuzhong，youduogebaoshouquheyuzhixiangjiandeduogekebianquzucheng。qizhongbaoshouquweisuoyouxijungongyou，kebianquyinxijunerbutong。jianyuci，16s rdnayichengweixijunjianbieyufenleiyanjiuzhongchangyongdebaxulie。
18s rdnashizhenheshengwujiyinzuzhongbianmahetangtidejiyin，qiyu5.8s rdnahe28s rdnajiyinzuchengyigezhuanludanyuan，sanzhegaodubaoshou，shiheyujiaogaodengjishuipingdeshengwuquntijiandexitongfenxi。zheigezhuanludanyuanqijiandejiangequweineizhuanlujiangequ（internal transcribed spacer, its），baokuoits1heits2liangbufen，youyujinhuaxiangduixunsuqiejuduotaixing，yinershiheyudengjishuipingjiaodidexitongxueyanjiu。
kuozengzicexushiduitedingzhangdudepcrchanwujinxingcexu，fenxixuliezhongdebianyi。16s/18s/itsdengkuozengzicexujitongguotiquhuanjingyangpindedna，tongguoheshidetongyongyinwukuozeng16s/18s/itsdemubiaoquyu，yiyanjiuhuanjingweishengwuduoyangxingjiqunluozuchengchayi。
anbiqideshengwuxinxituanduiweinintigongyizhanshidekuozengzicexujishufuwu，genjukehudeyanjiuxuqiu，liyonggaotongliangcexujishu，weinintuichuwanzhengdekuozengzicexujiejuefangan，xiezhuninyiqitansuoshengwuaomi。
我们的优势
chengshudecexupingtai：ce16s rdnaduovqu、18s rdnaheitsdenggaobianquyu，ce16sdanvquhuoshuangvqu
gaotongliang、gaozhiliangcexu：zhunquexinggao，fanyingwuzhongdezhenshiqingkuang
xiaolvgao、chengbendi：tongliangda、chanchushujuduo
biaozhunhuadefenxiliucheng：biaozhunfenxi、gaojifenxi、dingzhihuafenxi
fengfudekuozengzicexujifenxijingyan
dingzhihuadekuozengzicexufangan：kegenjushijixuqiuzhidinggexinghuadekuozengzicexufangan
技术流程
数据分析
样本要求
| 样本类型 | 总量 | 浓度 | OD260/OD280 |
| 组织样品 | >1.5g | ||
| 基因组DNA | ≥200ng | ≥6ng/μl | ≥ 1.8 |
| PCR产物 | ≥1μg | ≥10ng/μl |
技术参数
| 测序平台 | 测序策略 | 数据量 | 运转周期 |
| HiSeq MiSeq | PE250 | 根据具体项目情况而定，不低于合同约定的数据量 | 30-55个工作日 |
询价及订购
ganxieninxuanzeanbiqi16s/18s/itsdengkuozengzicexufuwu。ruyourenhexiangguandewenti，qingninlianxiwomendegongzuorenyuan，womenjikeweininjiedahefuwu。
参考文献
wuchter c, banning e, mincer t, et al. microbial diversity and methanogenic activity of antrim shale formation waters from recently fractured wells [j]. frontiers in microbiology, 2013, 4: 367.
cheng m, qian l, shen g, et al. microbiota modulate tumoral immune surveillance in lung through a γδt17 immune cell-dependent mechanism [j]. cancer research, 2014, 74(15): 4030-4041.
zhao l, wang g, siegel p, et al. quantitative genetic background of the host influences gut microbiomes in chickens [j]. scientific reports, 2013, 3.
