beijinganbiqishengwukejiyouxiangongsidedasskangtianjisuancexufuwu，tongguoniaoqiangfacexuyukangtixulieshujukuxiangjiehe“database assisted shotgun sequencing”（dass）dejishu，liyongxianyoujishu，keyijinxingkangticexufuwu。dasskangticexupingtaikeyitigongmianyiqiudanbaia（iga）kangticexufuwu。

igazaizhengchangrenxueqingzhongdehanliangjinciyuigg，zhanxueqingmianyiqiudanbaihanliangde10%-20%。igafenxueqingxinghefenmixingliangzhong，xueqingxingdeigasuiyouiggheigmdemouxiegongneng，kejiedaodiaolitunshiadcczuoyong，danzaixueqingzhongbingbuxianshimianyigongneng。fenmixingigashijitizhanmofangyuxitongdezhuyaochengfen，cunzaiyufenmiyezhong，rutuoye、leiye、churu、bihezhiqiguanfenmiye、weichangye、niaoye、hanyedeng。fenmixingigashijitizhanmojubukangganranmianyidezhuyaokangti,tanengyizhiweishengwuzaihuxidaoshangpifuzhe，jianhuanbingdufanzhi，shizhanmozhongyaopingzhang，duimouxiebingdu、xijunhe—bankangyuanjuyoukangtihuoxing，shifangzhibingyuantiruqinjitideshouyaofangxian，guyouchengzhanmojubukangti。igabunengtongguotaipan，xinshengerxueqingzhongwuigakangti，dankecongmuruzhonghuodefenmixingiga，duixinshengershijiaoweizhongyaodeyileikangti，zaixinshengerchusheng4～6geyuehou，xuezhongkechuxianiga，yihouzhujianshenggao，daoqingshaonianqidadaogaofeng。

zidasskangticexujishupingtaituichuyilai，yiduishushigemianyiqiudanbaia（iga）jinxingkangticexu。kehuzhixuyaotigong50-100 µgdeigayangpin，biankededaogaikangtideanjisuanxuliexinxi。