tongguoniaoqiangfayukangtishujukuxiangjiehede “database assisted shotgun sequencing（dass）”jishu，beijinganbiqishengwukejiyouxiangongsikeyiweinintigongmianyiqiudanbaim（igm）kangticexufuwu。

mianyiqiudanbaim（immunoglobulin m，igm）zhuyaoyoupizanghelinbajiezhongjiangxibaofenmihecheng，fenweiigm1heigm2lianggeyaxing，you5gedantitongguoyigejlianheerliujianlianjiechengwujuti，chendianxishushi19s，juyougaoqinheli，shimianyiqiudanbaizhongxiangduifenziliangjiaodade，wei970kda，yechengweijuqiudanbai。zaishengwujinhuaguochengzhongigmshishouyaochuxiandemianyiqiudanbai。zaigetifayuguochengzhong，wulunshibxibaomobiaomianig，haishihechengfenmidaoxueqingzhongdeig，igmdoushishouyaochuxiandeig，zaipeitaifayuwanqidetaierjiyounenglichanshengigm。zaikangyuancijiyoudaotiyemianyiyingdaguochengzhong，igmyexianchansheng。

fig. 1 igmjiegou

igmzhanxueqingmianyiqiudanbaizongliangde6%，yibanweibaohuxingkangti，juyoumianyixing，zhuyaocunzaiyudongwuxueyezhong，shijitishoukangyuancijihouxianchanshengdekangti，juyouqiangxibaoduhuoxinghexibaorongjiehuoxing，youyuigmzhuyaocunzaiyuxueguannei，shikangxueguanneiganrandeshouyaoxiankangti，duifangzhibaixuezheng、junxuezhengqizhongyaozuoyong。yinciigmkangtideceding，duimouxiechuanranbingrujiaxingganyanyoujiaogaodelinchuangzhenduanjiazhi。

igmkangtishimianyiyingdazhongfenmidekangti，yijingganrankuaisuchansheng，jingguoyiduanshijian，igmkangtiliangzhujianjianshaoerxiaoshi。tamenzaiyukangyuanjiehehouqidongbutidejilianfanying。igmyuruqinzhexianghujiehe，lianjieqilaijuchengyidui，kebeijushixibaodetunshi，dadaoqingchuruqinzhedemude。mutizhongdeigmbunengtongguotaipan，ruguotaierhuoxinshengerdexueyezhongfaxianyouigm，shuomingyifashengguogongneiganran。

huodegaoteyixingheqinhexingdeigmkangti，duiyuhenduojibingjinxingyouxiaodekangtizhiliaoyouzheyuelaiyuezhongyaodeyingxiang。anbiqishengwukeyigenjukehuxuyaotigongigmkangticexufuwu。