AbSeq™抗体测序

大发平台抗体技术

beijinganbiqishengwukejiyouxiangongsidekangticexupingtai，caiyongdass（database assisted shotgun sequencing）jishu，jiehexianyoujishudeyoushi，keduikangtijinxingfenxi。tashijiyuzhipujishudazaodekangticexupingtai。chulenenggouduikangtixuliezai10gegongzuorineiwanchenglixingcexuwai，zheixiangjishuhaishiyongyuqitayaxinghebutongxingshidekangti，ru：igm，yingguangouliankangti，gudinghuakangti，butongwuzhongdekangtideng。

beijinganbiqishengwukejiyouxiangongsizuoweikangtidingzhifuwugongyingshang，zhuanzhuyudankelongkangtideshengchanhegongchenglingyu。liyongduozhongkangtiwenkujishu（baokuoshijuntizhanshi、xijunzhanshihejiaomuzhanshi）yijizajiaoliujishu，shixianduiren、hou、tu、yang、ji、gou、luotuo、yangtuo、niu、xiaoshu、dashu、tunshujishayudedankelongkangtishengchan。ciwai，zaikangtidaguimozhibeifuwufangmian，nengzhibeiliyongxijunshengchandedanliankangti、shuanggongnengkangti、chuanlianscfv、miniantibodyhefab，yijiburudongwuxibaobiaodaminibody、qianhekangtiheigg。

一、平台特点

beijinganbiqishengwukejiyouxiangongsicaiyongdass（database assisted shotgun sequencing ) jishu，tongguoshiyongzhipuyiheshujuchulisuanfa，shengchengdaliangxuliexinxi，jineryunyongjisuanchengxuduims/msguangpucongtoucexu，liyongqineibushujuwajuegongjushidewomencongms/msguangpushujuzhongtiqumubiaokangtixuliexinxi。

AbSeq™抗体测序

运用DASS技术对V、J、C片段测序

muqianshijieshangdeyixieshujuku（runcbi）suiranbaohankangtiv、j、cpianduanxulie，danshizaikangtichengshudeguochengzhong，yigebxibaozhongdekangtijiyinyaojinglitixibaotubiandeguochengcaihuichanshenggaoqinhelidechengshukangti。wosidejisuanfangfarongcuolvgao，bingnengpipeixiangyingwuzhongdetubiantaiduan。shidetaiduandouhuibeijiancedao，erqiemeiyigeanjisuanweidiandouhuishengcheng20-27tiaoms/msguangpu，yinci，duiyunancexudegaofuansuan（proline）hejingansuan（arginine）taiduanyekeshixianceliang，erqiebuxuyaozaijinxingewaideedmancexulaifuzhucexu。

CDR3区测序

抗体轻链的 CDR3 区多由胚系基因编码，而重链的 CDR3 区则由 D 基因编码。D基因大部分区域由于受到核酸酶及末端转移酶等重组酶的作用而发生基因重排，导致抗体重链 CDR 区在数据库中无法进行同源比对。一般来说，一个成熟的抗体只有 1-4 个氨基酸是由 D 基因编码的，而其他序列都是基因重组排的结果，而只能对其进行 de novo 测序。

tongguoduozhongmeiqielaichanshengduozhonghuxiangzhongdiedetaiduan，yicedejiaozhangdeweizhianjisuanxulie。gaozhiliangdezhipushujuyugaoxiaolvdeshujuwajuesuanfadejiehe，shiwomenduikangticdr3 quyudejiedugengweiyouli，zuizhongkeyicedeshujukuzhongwutongyuanxuliede，20kdadedanbai。

等重氨基酸测序

与其他基于MS测序方法不同的是，此技术平台可以分辨大多数等重氨基酸复合物。
如：W 可从GE、AD、SV中区别出来（MS）；
R 可从GV中区别出来（MS）；
Q 可从GA、K中区别出来（Fragment spectra 和 MS）；
n kecongggzhongqubiechulai。

AbSeq?抗体测序 二、平台优势

全序列覆盖：

chuantongdekangticexujishunanyizuodaoquanxuliefugai，chuantongdejishujihudoudejiezhubufenshujukuzhongdexinxi。ji，shiceyibufen，yuceyibufen。erdasspingtaikeyidachengquanxuliefugai，duiyukangtidekebianqugengshimeigeweidianzhishaofugai4ci。

适用于超变区的数据处理算法

DASS采用质谱软件PEAKS Mass Spectrometry Software，与2008年Nature杂志发表的de novo单抗鸟枪法测序（Bandeira et al, 2008）zhongsuoyongdesuanfaxiangbi，dassnenggouduikangtidechaobianqujinxingjianding。

区分分子量接近的氨基酸

womencaiyongorbitrap elitecexuyi，qikeyitupochuantongcexujishudailaidefenbianlvdidexianzhi，keyitongshipipei3800getaiduan。chuantongjishuhennanqufenfenziliangjiejindeanjisuan（ruguanxiananhelaiansuanfenziliangchayijinwei0.03d），erdasszekeyikefuzheixieyouchuantongjishudailaideduanban。dassyijingchaoguomaldi-tofshijianfeixingzhipudejingdu，zhengchengweicongshikangtiyanjiuhekangtiyaowuyanfadekexuejiamendexinchong。

可对荧光标记单抗、IgMs、及其他非传统抗体进行测序

dasstongchangbushouxiaojituan（rufitc、biotin、alexa）deyingxiang，suiranjiaodadedanbaijituanhuijicexudailainandu，danruotigongjiaogaonongdudedanbairengkechenggongcexu，yinweicexuzhiliangyutigongdeyangbenliangxiangguan。

duiigmdecexufangfayu igg leisi，zhishixuyaodeyangbenlianggengduo。
dassshiyongyuxiaoshuyuanhetuyuankangti，ciwaimuqianyijingyoudayu50gelaiyuanyucangshuheyangdekangtiyebeichenggongcexu。
huaxuehuozheshengwuouliandekangtiyekebeichenggongcexu，zhishixuyaodeyangpinlianggengda。
许多杂交瘤细胞产生2条轻链，其中一条为无义链，也可对这些抗体混合物进行测序。

AbSeq?抗体测序 三、服务优势

可定制抗体片段测序

womenkegenjukehuxuqiu，kexuanzezhicedingkangtideqinglian、zhonglianhuozhekebianqu。lingwai，ouliandekangtiheigmdengshaojiankangtiyaxingdecexuyedoukedingzhi。

时间花费低

jishugexindailaideshishijianchengbendejiangdi，dasspingtaideshijianhuafeishichuantongcexufangfadeyibanyixia，zaishoudaoyangpinhou，3-10gegongzuorineijikewanchengquanxulieceding。

样品量要求低

jinxuyaotigong25-100μgchunhuadekangti。

测序种类多样

keduiyingguangbiaojidankang、igms、jiqitafeichuantongkangtijinxingcexu。

四、服务保障

fugai vjc he vdjc quyu。
meigeanjisuanweidianzhishaofugai4gebutongdetaiduan。
duiqingliandecexuwuchafanweizai+/- 1.2 da，duizhongliandewuchafanweizai+/- 1.8 da。

五、样品要求

普通 IgG 和偶联修饰基团（FITC，Alexa，Biotin）的 IgG：需要 25-100 μg 样品，如能提供 200 μg样品效果会更佳。
hanyou bsa dedankang，yangyuanhuozhetuyuan，igm，gudinghuadedankang，yijiouliandaxiushijituan（pe，apc）dedankang：xuyaojingguoteshuyangbenchuli，yiban 500 μg yangpinkemanzucexu。