北京安必奇生物科技有限公司可提供多种拟南芥（Arabidopsis thaliana）yichuanzhuanhuatixi，yimanzukehudebutongyanjiuxuqiu：rujiyingongnengfenxi、jiyinbiaoda、qidongzihuoxingpingguyijidanbaizhi-danbaizhihuodanbaizhi- dnaxianghuzuoyongyanjiudeng。
ninanjieshizhiwulingyuyingyongjiaoweiguangfandemoshishengwu。youyujuyoujiaoxiaodejiyinzu（135 mbp），shengmingzhouqiduan，keyizaiduozhonghuanjingxiashengzhang，yizhibeishiweiyanjiuzhiwuyichuanxue、jinhua、zhongqunyichuanxuehezhiwushengzhangfayudexitong。
农杆菌介导的蘸花法是目前相对成熟、应用较为广泛的拟南芥稳定遗传转化方法。转化步骤大致如下：拟南芥（T0代）的花序浸没在一定浓度的含有目标转化质粒的根癌土壤杆菌（Agrobacterium tumefaciens）（changyongdegenainongganjunjunzhuweigv3101）xuanfuyezhong，ranhoufangzaiyidingtiaojianxiajinxingpeiyang（tu1 a-e）。daizhiwuchengshuhoushoujit0daizhiwudezhongzi，jiangzheixiezhongzifangzaihanyoutedingkangshengsudepeiyangjishangjinxingshengzhangshaixuan（tu1f），huodeyangxingzhizhu。yuqitazhiwuzhuanhuafangfaxiangbi，zhanhuafaxuyaojiaoshaoderenlihezhuanyongshiji，suoxushebeixiangduijiandanqiezhuanhuaxiaolvjiaogao（zaiyouhuadetiaojianxiadayu1%）（1）。
tu 1. ninanjiezhanhuafajianyaoguocheng（2）。
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参考文献：
Ghedira R.; et al. (2013) The efficiency of Arabidopsis thaliana floral dip transformation is determined not only by the Agrobacterium strain used but also by the physiology and the ecotype of the dipped plant. Moecular Plant Microbe Interaction, 26(7), 823-832.
Zhang, X.; et al. (2006). Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nature Protocols, 1(2), 641-645.
