anbiqishengwuzuoweijiyinzubianjifuwutigongzhe，zhuanzhuyuliyongcrispr/cas9jishuhegaotongliangshaixuanjishutuidongjichukeyanheyiyaoxingyedebuduanfazhan。womenyongyouzhuanyedejishutuanduihewanshandejiyinzubianjipingtai，duonianlaizhiliyuweikehutigongsgrnashejiyuyanzheng、zaitigoujian、jiyinqiaochu、jiyinqiaoru、jiyindingdiantubianyuxiufu、tuobaxiaoyingfenxihegaotongliangsgrnawenkushaixuandengquantaojiyinzubianjijiejuefangan。

guilvchengcudejiangeduanhuiwenzhongfuxulie(clustered regularly interspaced short palindromic repeats, crispr)shixijunheguxijunweiyingduibingduhewaiyuanzhilidegongjiyanhuaerlaidehuodexingmianyifangyujizhi。crispr/cas9xitongyousgrna（single-guide rna）hecas9danbailianggezuoyongyuanjianzucheng，sgrnayindaocas9danbaishibiebingqiegejiyinzutedingweidianxingchengshuanglianduanlie（dsb, double strand break），xibaosuizhiliyongfeitongyuanmoduanlianjie（nhej）hetongyuanzhongzuxiufu（hdr）duishuanglianduanliejinxingxiufu。feitongyuanmoduanlianjieshiyizhongyicuoyitubiandexiufutujing，rongyidaozhiduanlieweidiancharuhuoqueshishugejianji，congeryinqimubiaojiyinyimatubianhuotiqianzhongzhi；tongyuanzhongzuxiufuzexuyaoyinruwaiyuanxiufumoban，shixianjingzhundejiyincharuhetihuan。

crispr/cas9jishucaozuojiandan、bianjigaoxiaojingzhun、kejinxingduozhongbaxiangxiushi，yichengweijixinzhihesuanmei（zfn）jizhuanlujihuoyangxiaoyinghesuanmei（talen）zhihoudedisandaijiyinzubianjigongju，bingguangfandeyingyongyurenleixibao、dashu、xiaoshu、banmayu、guoying、shuidao、xiaomai、ninanjiedengdongzhiwuyijiweishengwudejiyinzubaxianggaizao。anbiqishengwujingyanfengfudeyanfatuanduiyunyongcrispr/cas9jishujiqiyanshengdedcas9xitonghedanjianjibianjixitong，yangeanzhaokehudeyaoqiu，wanchenggezhongjiyinqiaochu、jiyindingdianqiaoruyutihuan、danjianjibianji、jiyinjihuoyuyizhi、jiyinbiaoguanxiushidengxiangmu。

服务内容

• 高通量sgRNA文库筛选服务：qiaochuwenku，jihuowenku，yizhiwenku。

• 基因编辑细胞系定制服务：jiyinqiaochuxibaoxi，tiaojianqiaochuxibaoxi，dingdiantubianxibaoxi，jiyinqiaoruxibaoxi。

• 脱靶效应分析服务：quanjiyinzucexu，guide-seq，circle-seq。

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参考文献：

• ran, f. a., et al. (2013) genome engineering using the crispr-cas9 system. nat protoc 8, 2281-2308.