crispr-cas9shijizfn、talensdengjiyinbianjijishutuichuhoudedisandaijiyinbianjijishu，yinqixiaolvgao、jianbian、chengbendi，yichengweidangjinzhuliudejiyinbianjijishuzhiyi。gongchenghuadecrispr/cas9 xitongyouliangbufenzucheng：juyoudaoxianggongnengdesingle guide rna (sgrna)hexingshidnaqiegegongnengdecas9 hesuanneiqiemei。dangxibaoneitongshibiaoda cas9 danbaihe sgrnashi，cas9danbaijiehesgrnabaxiangdaomudednaxulie，youdaodnashuanglianduanlie (dsb)，yinfaxibaoneibudednaxiufujizhi：（1）ruguoyoudnaxiufumobanjinrudaoxibaozhong，dsbyijuxiufumoban，tongguotongyuanzhongzuxiufu（hdr）tujingduiduanliednajinxingxiufu，congershixianjiyinqiaoru、tihuanhuodiantubian；（2）dangweitigong dna xiufumobanshi，xibaohuitongguofeitongyuanmoduanlianjiefangshi（nhej）duiduanliednajinxingxiufu，xiufuguochengzhongtongchanghuifashengjianjicharuhuoqueshidecuopeixianxiangzaochengyimatubian，congershixianjiyinqiaochu（knock-out）。 chulechuantongdespcas9，spcas9-hf1、sacas9dengqitabanbendecas9xitongyeyiguangfanyingyongyujiyinbianji。

anbiqiyouduoniandewendingxibaoxigoujianjingyan，keyigenjunindekeyanxuqiujiang cas9hesuanmeijiyinwendingzhenghedaoninsuozhidingdesuzhuxibaojiyinzu。

应用领域

• sgrnagaotongliangshaixuan

• jiyinbianjilixiangdexibaomoxing，baokuojiyinqiaochu、jiyinqiaoru、jiyinyoubian、jiyinbiaojideng

• 基因功能的研究

我们的优势

• yongyouchengshudewendingxibaoxigoujianpingtaiheyouxiudejishutuandui

• linghuodexibaoxigoujiancelve：zhizhitizhuanranfa、dianchuankongfahebingduzhuandaofa

• yangedezhiliangkongzhitixi：xibaoxiwuxijun、zhenjunhezhiyuantiwuran

• fenlidankelongxibao，baozhengdanyijiyinbeijingxiachixu、gaoshuipingdebiaodacas9danbai

• cas9 wendingzhengheshisgrnagongzhuanranhuogongzhuandaogengyicaozuo

• yongt7 endonuclease iduicas9huoxingjinxinggongnengyanzheng

• fengfudesuzhuxibaoleixing：ganaixibao、ruxianaixibao、jiechangaixibao、feiaixibao、yixianaixibao、weiaixibao、peitaishenxibaodeng

服务流程

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案例分析

案例1：HEK293T-SpCas9细胞系T7EI检测结果