wendingxibaoxizaijiyingongnengyanjiu、zhongzudanbaizhibei、yaowushaixuanjibadianyanzheng、zhongliuzhiliaodengshengwuxueyanjiuzhongfahuizhongyaozuoyong。anbiqishengwuzhuanyedeyanfatuanduiyongyoufengfudexibaoxigoujianhecrispr/cas9jiyinbianjixiangmujingyan，zhiliyuweiguangdakehutigongdingzhidejiyinbianjixibaoxigoujianfuwu。womendefuwuhangaijiyinqiaochuxibaoxi、tiaojianqiaochuxibaoxi、jiyinqiaoruxibaoxi、dingdiantubianxibaoxi、jiyinjihuo/yizhixibaoxi。jiyugaoxiaowendingdexibaozhuanranxitonghejiyinbianjixitong，womenchenggongzaibutongxibaoxishixianjiyinbianji，baokuodanbuxianyuyizhuanranxibaohuonanzhuanranzhongliuxibao，shenjingxibao，ganxibaoheqitaxibao。

服务内容：

• 基因敲除细胞系

anbiqishengwutigongdanjiyinqiaochuxibaoxi，duojiyinqiaochuxibaoxihedapianduanshanchuxibaoxidingzhifuwu。

• 条件敲除细胞系

jiyindebiaodajuyoushikongteyixing，duitineiguanjianjiyinjinxingqiaochuhetihuandengfangshidejiyinbianji，wangwangdaozhidongwupeitaisiwang，zuaijiyindegongnengyanjiu。tiaojianxingqiaochukeyishimubiaojiyindebiaodahuoqueshifashengzaidongwufayudemouyitedingjieduanhuomouyitedingzuzhiqiguan。anbiqishengwujiangcre-loxpzhongzuxitongyucrispr/cas9jishuxiangjiehe，weiguangdakehutigongwendingdetiaojianqiaochuxibaoxi。

• 基因敲入细胞系

womendejiyinqiaoruxibaoxibaokuobaogaojiyinqiaoruxibaoxihekehuzhidingjiyinqiaoruxibaoxi，shiyongyouhuahoudecrispr/cas9xitong，womenchenggongweikehuwanchengdapianduanjiyinqiaoruxiangmu。

• 定点突变细胞系

liyongcrispr/cas9jishu，tongguoyinrutedingdexiufumoban，shixianduimubiaojiyinjianjixuliededingdianxiushi，baokuojianjidetianjia、shanchuhetihuan。

• 基因激活/抑制细胞系

anbiqideliyongdcas9-vp64/dcas9-krabjiyinwendingbiaodadedankelongxibaozhu，tongguojiangqidongzibaxiangdesgrnadaorudcas9-vp64/dcas9-krabwendingxibaozhu，congershixianbaxiangdeneiyuanjiyinjihuo/yizhi。

定制基因编辑细胞系构建服务流程：

• xiangmupinggu：pinggumubiaojiyinqiaochu/qiaorushifoujuyoukexingxing，yushiyanjiancebaxibaozhuanranxiaolvhedankelongshengzhangnengli。

• sgrnashejiyuzaitigoujian：shejiduogesgrna，goujianzaiti，tongguoxibaoshiyanshaixuanbianjixiaolvjiaogaodesgrna。

• xibaozhuanran：jiangcrisprzaitihuornpliyongdianzhuanfahuobingduzhuanranfadaorushoutixibao。

• dankelongshaixuanyuyanzheng：cexuyanzhengjiyinshuipingqiaochu(morenfangfa)，facshewbyanzhengdanbaishuipingqiaochu(kexuan)。

• jiaofuwendingxibaoxi：zhiyuantijiancewuwuranhou，jiaofujiyinbianjixibaoxi。

交付内容

• wendingxibaoxi（dankelonghuoxibaochi）

• 项目报告

我们的优势

• shiyongyouhuadecrispr/cas9xitong，bianjixiaolvgao，tuobaxiaolvdi。

• duozhongfangangongxuanze，dingzhihuafuwumanzubutongdeyanjiuxuqiu。

• dingqihuibaoshiyanjindu，shiyanguochengtouminghua。