bingdubaozhuang(virus packaging),shiyizhongjiyinzhenduan、jiyinzhiliaojishu。tongguojiangwaiyuanjiyinbaozhuangdaotianranbingdudewaiqiaozhong,liyongbingduduimudexibaodeganranxing,jiangwaiyuanjiyindaorumudexibao,congergaibianwaiyuanjiyinzaixibaozhongdebiaoda。muqianzhuliudebingduzaitixitongzhuyaobaokuo:
manbingdu(lentivirus): shinizhuanlubingdudeyizhong,nenggouganranfenlieqihefeifenlieqidexibao,congerhenizhuanlubingduqubiekailai。yinweikeyizhenghedaoxibaojiyinzudetexing,beiguangfanyingyongyuxibaoganran、dongwushiyanhecar-txibaozhiliao;
xianxiangguanbingdu(adeno-associated virus): shiyileijiegoujiandan、wubaomodedanliandnaquexianxingbingdu。yanjiuzhongcaiyongdezhongzuxianxiangguanbingdu(recombination adeno-associated virus, raav)queshaozhenghehefuzhibixudejiyineryouliyuxibaojiyinzu,qisuzhufanweiguang、kuosanxingnengliqiang、tineibiaodashijianzhang、gaoanquanxinghedimianyiyuanxing、zhongleiduoyangyijingchengweikeyanhejiyinzhiliaozhongjushichangqianjingdebingduzaitizhiyi;
xianbingdu(adenovirus): shiyizhongwubaomodednabingdu,qisuzhufanweiguang、baozairongliangda,yiyudaguimoshengchan,beiguangfanyingyongyuxibaoganran、yimiaokaifahejiyinzhiliao;
nizhuanlubingdu(retrovirus): zuidatedianzaiyuqiteyixingganranfenlieqixibao,yincishiyongyuganxibaoxiangguandedaliangyanjiu,rushenjingganxibaoyanjiu、zaoxueganxibaoyanjiu。
病毒表达系统 | 慢病毒 | 腺相关病毒 | 腺病毒 | 逆转录病毒 |
包膜 | 有 | 无 | 无 | 有 |
病毒基因组 | ssRNA | ssDNA | dsDNA | ssRNA |
是否整合 | 整合型:随机稳定整合 非整整合型:不整合 | 不整合 | 不整合 | 随机整合并稳定遗传 |
感染细胞类型 | 分裂和不分裂细胞 | 分裂和不分裂细胞 | 分裂和不分裂细胞 | 分裂细胞 |
表达水平 | 适中 | 适中 | 高 | 适中 |
维持表达时间 | 长期稳定表达 | 稳定表达数月 | 瞬时表达 | 长期稳定表达 |
滴度 | 109TU/ml | 1013v.g./ml | 1011PFU/ml | 109TU/ml |
包装容量 | ~9kb,滴度随插入片段长度增加而降低 | ~4.7kb,滴度随插入片段长度增加而降低 | ~38kb,滴度随插入片段长度增加而降低 | ~8kb,滴度随插入片段长度增加而降低 |
感染细胞 | 适用 | 适用 | 适用 | 分裂型细胞适用 |
动物实验 | 滴度低,效率低 | 非常适合 | 适合 | 效率低 |
免疫原性 | 低免疫原性 | 极低免疫原性 | 高免疫原性 | 中免疫原性 |