jiyingongchengjishu，tebieshicrispr/cas9jiyinbianjixitong，tigaolekexuejiajinxingjiyinzubianjidenengli。raner，renmenjingchangdanxinzhuanjiyinshipindeanquanxing。yinci，kexuejiakaifaledna-freedejiyinzubianjifangfa。muqian，dna-freejiyinbianjifangfabujindedaolexueshujieguangfandeguanzhu，haidedaolegongyejiedeqinglai。
北京安必奇生物可提供多种DNA-free的植物基因组编辑方法，可帮助实现更安全的植物转基因产品。这些技术包括瞬时表达CRISPR/Cas9质粒DNA（transiently expressed CRISPR/Cas9 plasmids DNA，TECCDNA） （图1）、体外转录CRISPR/Cas9 （in vitro transcripts，ivts）yijiyouchunhuahaodecas9danbaihesgrnaszuchengdeyuzuzhuanghetanghesuanfuhewu（ribonucleoprotein， rnps）（tu2）。zheixiejishukeyibimianwaiyuandnayujiyinzudezhenghe，bingkeyijianshaotuobaxiaoying。ciwai，yuchuantongdejishuxiangbi，zheixiejishukebimianyongzajiaohuohuijiaolaifenlicrispr/cas9qianheti，yincigengbianyi、shiyanzhouqigengduan。
图 1. 瞬时CRISPR/Cas9 DNA 编辑技术（Zhang et al., 2016）。
图 2. DNA-free植物CRISPR/Cas9 RNAs体外转录和RNP技术（Yin et al., 2017）。
womenketigongfangfajiangteccdna、ivtshernps zhuanruzhiwuxibao，zhuyaoyoujuyierchun(peg)、yanglizizhizhihe/huodianchuankongjiedaodeyuanshengzhitizhuanhuahejiyinqiangjiedaodeweichengshupeizhuanhua（tu1he2）。gaijishuyunyongdaoninanjie、yancao、shengcai、shuidaohexiaomaidengwuzhong。womenyongyoudezhuanjia，keyiweikehutigongcongteccdna、ivtshernpsdezhibeidaotubianshaixuandefuwu。ruxujinyibudexinxi，qingsuishiyuwomendegongzuorenyuanlianxi。
参考文献：
1. Zhang Y.; et al. Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA. Nature Comm. 2016, 7: 12617.
2. Yin K.; et al. Progress and prospects in plant genome editing. Nature Plants. 2017, 3: 17107.
