dnajiajihuashizaijiajizhuanyimeidecuihuaxia，dnadecglianggehegansuandebaomidingbeixuanzexingditianjiajiaji，xingcheng5-jiajibaomiding，zheichangjianyujiyinde5'-cg-3'xulie。dnajiajihuashizhongyaodebiaoguanyichuanxuebiaojixinxi，zaijiyinbiaodadiaokong、yichuanyinji、ransezhizhongsu、xijunsuzhufangyu、peitaifayuyijirenleiaizhengfashengdengfangmianzhongqizhezhongyaodezuoyong；huodequanjiyinzufanweineisuoyoucweidiandejiajihuashuipingshuju，duibiaoguanyichuanxuedeshikongteyixingyanjiujuyouzhongyaoyiyi。

quanjiyinzudnajiajihuacexu（whole genome bisulfite sequencing，wgbs）shicaiyongzhongyaliusuanyanchulidnashiqiweijiajihuaxiushidebaomidingczhuanhuaweiniaomidingu，tongguoduichulihoudednajieheilluminagaotongliangcexupingtaijinxingquanjiyinzuzhongcexu，bingyucankaojiyinzujinxingbidui，zaiquanjiyinzushuipingshangshixiandanjianjifenbianlv，jingquepinggudange c jianjidejiajihuashuiping，jinergoujianjingxidequanjiyinzudnajiajihuatupu。wgbsbeiguangfanyingyongyurenleijibingyudongzhiwuyanjiuzhong，ruxibaofenhua、zuzhifayudengjichujizhiyanjiu，yijidongzhiwuyuzhong、renleijiankangyujibingzhiliaodengyanjiulingyu。

我们的优势

• kuaisu、jingzhunzhaodaochayijiajihuaquyu

• jiancefanweiguang，shixianquanjiyinzushuipingshangjiajihuayanjiu

• 稳定高效的项目流程

• zhouqiduan，youjingzhenglidejiageyoushi

技术流程

数据分析

标准分析

• 原始数据质控

• 比对到参考基因组

• 甲基化位点识别

• jiajihuashuipingfenbuqushi：cpg、chg、chhjiajihuafenbubili；chg/chhjiajihuacfujin9bpdexuliefenxi；ransetishuipingjiajihuamidufenbu；quanjiyinzujiajihuatupu；butongjiyinzuquyu/jiyinyuanjianjiajihuafenbu

• duoyangbenjiajihuachayifenxi：juleifenxi；gofenxi；keggfenxi

高级分析

• chayibiaodajiyindejiajihuashuiping

• chayibiaodazhuanzuozidejiajihuashuiping

• jiajihuaxiushiyubiaodadiaokongguanxi

• jiajihuajiyinhefeijiajihuajiyindebiaodafenxi

样本要求

技术参数