muqian,16s/18s/its rrnashiweishengwufenleijiandinghexitongfayudezhibiao。yuanheweishengwude16s rrnajiyinzhangduyuewei1500 bp,baohan10gebaoshouquhe9gegaobianqu(v1-v9),tongshijuyougaodudebaoshouxingheteyixing,baoshouqukefanyingjunshujiandeqinyuanguanxi,kebianqutixianjunshuzhijiandechayi。zhenheweishengwu18s rrnajiyinzhangduyuewei1500~2000 bp,xuliezhongyejiyoubaoshouquhekebianqu,youyujinhuasulvbijiaobaoshou,shiyongyuzhongjiyishangdefenleijianding。zhenheweishengwuitszhangduyuewei500 bp,zaizhongneibutongjunzhuzhijiangaodubaoshou,erzaijunzhongjiancunzaijiaodadebianhua,biaoxianchujidadexulieduotaixing,beiguangfanyingyongyushengwuzhongjianxitongfayuheyanjiu。
jiwangde16s/18s/its rrnakuozengzicexuzhenduiqixuliededangehuolianxudelianggehuosangekebianqushejiyinwukuozengzaicexufenxi;erxianzaijiyusandaipacbio smrtcexupingtai,womenshiyong27f/1492rdeyinwu,pingjunduzhang10~18 k,keqingsongfugai16s/18s/its rrnaquanzhangxulie,tupoerdaicexuduzhangduandejuxianxing,shixianjingquedao“zhong”defenleijianding,kezaiduogelingyuyingyongruwuzhongjinhua、wuzhongfengdu、huanjingyushengtai、yixuelingyudeng,yanjiubiaopiweishengwudeguanxi、rentichangdaoweishengwuyujibing。
我们的优势
chengshudecexupingtai:pacbio smrtxitong
高质量的数据保障
gaozhunquedejianding,ketigongduozhongccs readsshumudexuanze
丰富的项目经验
分析内容多样
zhouqiduan,youjingzhenglidejiageyoushi
技术流程
数据分析
womenshiyongcircular consensus sequencing(ccs)desuanfalaijiaozhengcexuchanshengdecuowu,tigaocexudezhunquexing。tongguoquchubarcodeyijizhangduxiaoyu150 bpyijimolengliangkedexulie,tongguo99%dexiangsixingchanshengotuhebiduishujukuzuihoujinxingfenlei。
样本要求
样本类型 | 总量 | 浓度 | OD260/OD280 |
基因组DNA | ≥200 ng | ≥10 ng/μl | ≥ 1.8 |
PCR产物 | 电泳条带单一,大小在1~2 Kb |
技术参数
测序平台 | 文库类型 | 数据量 | 运转周期 |
PacBio SMRT | 2 K | 每个样本约3000~40000个CCS reads | 视具体项目情况而定 |