crlspr/cas9jishushijiyinbianjilishishangdeyouyilichengbei。gaijishutongguogrnazhidaocashesuanmeiduibaxiangjiyinjinxingtedingdnaxiushi。grnawenkushiyaowushaixuanhuobaxiangshaixuantedingtongludelixianggongju，grnawenkudejianlijiangzaigongnengjiyinshaixuan、jibingjizhiyanjiujiyaowuyanfadengfangmianfahuizhongyaodegongneng。

安必奇生物可为客户定制针对特定基因分类的。我们提供的gRNA文库包括：全基因组文库、 lncRNA 文库、信号通路、细胞凋亡、细胞增殖、离子通道、核受体相关、各种疾病相关等文库。除了可以提供Plasmid –gRNA Library以外，还可以根据客户需要对gRNA文库进行病毒包装，yipackaged viral -grna libraryxingshitijiao。womenxuanzedejiyinbadianjingguoduogejiaohushujukushaicha，shejidegrnajunjingguoqiaochuxiaolvyanzheng。womenhuiweinintigonggrnawenkugoujianfuwu，jiaofujininzhuanranjibiedewenku，kezhijieyongyugaotongliangyaowubadianyijigongnengjiyindeshaixuan。

全基因组gRNA文库的构建

anbiqishengwudequanjiyinzugrnawenkudegoujiankeyizhenduiduozhongleixingdejiyinzudna，baokuoorf cdna，lncrna cdnayijitedingquyudecdnapianduandeng。nenggouzhenduiquanzhangcdnanaizhijiyinzudnagoujiangaoxiaodegrnawenku，shiyongyugaotonglianggongnengjiyinjixiangguanyaowubadianshaixuan。

客户定制gRNA文库

anbiqishengwutigongdezidingyiwenkuyou：gezhongxinhaotongluxiangguanjiyindegrnawenkuyijijibingxiangguanjiyindegrnawenku。genjukehuquedingdeyanjiutongluhuojibing，shaixuanxiangguandebajiyin。womenweikehufenbieshejizheixiebajiyindegrnaxuliebinggoujiangrna library。

技术流程

1. kehutigongbajiyin/baxuliexinxi；

2. womenjinxinggrnawenkugoujian，chouyangcexuyanzheng；

3. genjukehuyaoqiu，goujiangrnabiaodazhiliwenku，yiplasmid –grna libraryxingshitijiao；

4. genjukehuxuyao，duigrnawenkujinxingbingdubaozhuang，yipackaged viral -grna libraryxingshitijiao；

5. jiangqiliantongchouyangbaogao、chanpinshuomingshuyiqijisongdaokehushouzhong。

我们的优势

• grnazizhushejijizaitigoujianjishu，juyougenggaoxiaolv、gengdichengbenyoushi；

• jiehemanbingdubaozhuangxitong，keyongyugezhongnanzhuanrandexibao，ruyuandaixibaohuoshenjingyuanxibaodeng；

• liushixibao、mianyiyingguangdengduozhongfenxuanfangfa，bianyuhouqixibaoshaixuan。

• weikehutigongconggrnawenkusheji、hecheng、zaitigoujian、manbingdubaozhuangdaoxibaofenxuandezhengtifuwu。

应用领域

• yongyutongluhuojibingxiangguanjiyindeshaixuan[1]；

• gaotonglianggongnengjiyinshaixuan[2]；

• yaowubadianshaixuan[3]。

参考文献
[1] Noorani I. 140 Genome-wide CRISPR/cas9 Knockout Screens in Human Glioblastoma Identify Genetic Vulnerabilities[J]. Neurosurgery, 2016, 63: 157.
[2] Zhu S, Li W, Liu J, et al. Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library[J]. Nature Biotechnology, 2016.
[3] Shalem O, Sanjana N E, Hartenian E, et al. Genome-scale CRISPR-Cas9 knockout screening in human cells[J]. Science, 2014, 343(6166): 84-87.

询价及订购

    ganxieninxuanzewogongsidejiyinkelongfuwu。ruyourenhexiangguandewenti，qingninlianxiwomendegongzuorenyuan，womenjinkuaiweininjiedahefuwu。