mianyigongchendian(co-immunoprecipitation,co-ip)jishushiyikangtihekangyuanzhijiandezuoyongweijichude,yongyuyanjiudanbaizhixianghuzuoyongdefangfa。yuanlishiyixibaoneiyuanxingbadanbaiweiyouer,jiangbadanbaikangtiyuxibaozongdanbaijinxinggongfuyu,cujinmianyifuhewudexingcheng。suihoujiarunenggouyubadanbaikangtijiehedeprotein a/g,xingcheng“jiehedanbai-badanbai-badanbaikangti”fuhewu。mianyifuhewujingchunhuahoujinxingningjiaodianyongfenli,jiehewestern blot huozhipujishu,jinerdadaochunhuamudedanbaizhi、dingxingjiancekangyuanhuokangtidemude。yuqitayanjiufangfaxiangbi,mianyigongchendianzaishenglitiaojianxiajiancedanbaizhizhijiandexianghuzuoyong,yinci,bujinkeyijiancedaotineixingchengdefuheti,erqiekepaichuguobiaodabadanbaisuodailaidejiayangxing。qicineiyuanxingdebadanbaizhishijiagong、xiushihechengshudedanbaizhi,yinci,yilaiyuxiushidedanbaizhixianghuzuoyongyenengbeijiancedao。
免疫共沉淀原理图
服务内容
• 标签蛋白的免疫共沉淀 (Myc-tag, Flag-tag, HA-tag)
• 非标签蛋白的免疫共沉淀 (Native protein)
• 染色质免疫共沉淀(Chip-IP)
技术流程
• 构建相关真核表达载体,转染细胞并获得表达;
• 蛋白质抽提与定量;
• 免疫沉淀(以normal IgG作为对照);
• 免疫沉淀产物SDS-PAGE和western blot分析;
• 技术服务实验报告提交。
客户需准备的材料
• 组织、细胞、蛋白质溶液等样品,待测蛋白的相关信息等;
• 如需构建基因的过表达载体,则还需提供过表达载体及其相关信息;
• 其他与免疫沉淀相关信息。
实验报告内容
• 免疫沉淀产物,电子图片及分析结果;
• 蛋白质鉴定结果,SDS-PAGE及western blot分析结果;
• 实验步骤、使用仪器等。
核酸与蛋白互作研究订单表
询价及订购
ganxieninxuanzewogongsidemianyigongchendianjiancefuwu。ruyouxiangguandewenti,qingninlianxiwomendegongzuorenyuan,womenjinkuaiweininjiedahefuwu。