xibaomianyiyingguangshigenjukangyuankangtifanyingdeyuanli，xianjiangyizhidekangyuanhuokangtibiaojishangyingguangsuzhichengyingguangbiaojiwu，zaiyongzheizhongyingguangkangti（huokangyuan）zuoweifenzitanzhenjianchaxibaohuozuzhineidexiangyingkangyuan（huokangti）。zaixibaohuozuzhizhongxingchengdekangyuankangtifuhewushanghanyouyingguangsu，liyongyingguangxianweijingguanchabiaoben，yingguangsushoujifaguangdezhaosheerfachumingliangdeyingguang（huanglvsehuojiehongse），keyikanjianyingguangsuozaidexibaohuozuzhi，congerquedingkangyuanhuokangtidexingzhi、dingwei，yijiliyongdingliangjishucedinghanliang。

anbiqishengwudefenzishengwuxue，xibaoshengwuxuechanpin，shiyantiaojian，shebei，shiyanrenyuan，zhulinindeshiyanwancheng。

技术流程 

1. xibaopapian：jiangxibaoan20%-30%dehuihedujiezhongdao24kongxibaopeiyangbanzhongdeyuanxinggaibopianshang。

2. 细胞固定：4%冷的多聚甲醛固定20分钟，PBS洗三遍。
   

3. xibaotongtou：triton x-100 tongtou20fenzhong，pbsxisanbian。

4. fengbi：yongyuerkangxiangtongsuzhudexueqingfengbi30fenzhong，pbsxisanbian。

5. yikangfuyu：yikang4dushiheneiguoye，yeke37du2xiaoshi，pbsxisanbian。

6. erkangfuyu：erkangshiwenfuyu2xiaoshihuozhe37du1.5xiaoshi（biguang），pbsxisanbian。

7. dapiranhe：zaierkangfuyu1xiaoshihoujiaruzhongnongduwei100ng/mldedapijixufuyu。ranhoukezaiyingguangxianweijingxiaguanchahuozhijiefengpianbaocun。

8. ganyoufengpian：zaizaibopianshangdiyixiaodiganyou，xiaoxindijiangxibaopapiancongxibaopeiyangbanzhongquchulai，zhongzhiyouxibaodeyimianchaoxiaqingqingdegaizaiganyoushang，bimianchanshengguoqipao。4dufuyuguoyelianggan。

服务流程
• 根据客户所需要目的蛋白和提供的一抗，进行预实验验证一抗的效价。
• 目的蛋白和一抗二抗孵育显色。
• 提供实验报告：包括详细的实验方法及实验结果的相关图表。
交付结果
完整的实验报告，包括实验过程、所用仪器试剂和相关分析数据
询价及订购

ganxieninxuanzewogongsidexibaomianyiyingguangjiancefuwu。ruyourenhexiangguandewenti，qingninlianxiwomendegongzuorenyuan，womenjinkuaiweininjiedahefuwu。