yuqitagongsicaiyongzajiaoliujishushengchantudankelongkangtidejishubutong，anbiqishengwujiyugongsideshijuntizhanshijishupingtai，goujianletudankelongkangtiyanfapingtai，keyiweikehutigongtudankelongkangtizhibeifuwu。

tukangtikushituyuanduokelongkangtihedankelongkangtidelaiyuan，chanshengdekangtiyongyouteyixing、huoxingheqinhelideyoudian。yinweituheniechileidongwuzhongchangyongdedashu、xiaoshujinhuaguanxijiaoyuan，niechileidankelongkangti（yijicongzhuanrulerentimianyiqiudanbaijiyindezhuanjiyinxiaoshuzhongzhibeiderendankelongkangti）duiniechileiherentikangyuangongtongdekangyuanjuedingcubuqizuoyong，ertukangtiquewangwangkeyibianshiniechileiherentikangyuangongtongkangyuanjuedingcu，kefulekangtishengchanzhongzheiyizhangai。shijuntizhanshijishuduibiaoxing（danbaizhi）hejiyinxing（cdna）tongshijinxingshaixuan，yineryoucifazhibeidetudankelongkangtijuyouyoushi。ciwai，hetuzajiaoliujishuxiangbi，womengongsideshijuntizhanshipingtaikeyitongshishengchangengduoshuliangdedankelongkangti。

噬菌体展示系统是在大肠杆菌中实现的，而兔Fab抗体片段对大肠杆菌却是有毒性的。通常有两种方法可以避免兔Fab抗体片段在细菌细胞中表达的毒性、不溶性以及表达量低的问题。一是链接兔源抗体可变区(Vλ,  Vk, and VH)  和人源抗体恒定区(Ck and CH1)，以此来制造兔人嵌合Fab抗体库。Carlos F. Barbas III 和 Christoph Rader博士的研究证明这种方法是有效的。另一种方法是利用酵母展示技术在酵母Pichia pastoriszhongbiaodatedingdetuyuanfab(huoscfv)kangti，womengongsimuqianbingbushiyongzheizhongfangfa。

zhidezhuyideshi，xiaoshuyijirendek  qingliandoujuyoulianggelianneierliujian，erdaduoshutuyuankangtichucizhiwaihaiyoulingyigelianjiekebianquhehengdingqudeerliujian。kexuejiazaiyanjiuzheitiaoduochulaideerliujianzaitu/renqianhefabkudejianzaoheshaixuanzhongdezuoyongshifaxian，juyoubastubianyijiyeshengqinbenb9zheilianggeyixingdoushizhibeituyuandankelongkangtidelaiyuan。

anbiqishengwuyijingweikehugoujianleduogetumianyikangtiku，bingweiwomendekehutigongtexingkangyuandekangtishaixuanfuwu。zhidezhichudeshi， pcryinwuhechengzhongyijipcrfanyingzhongdecuowuzaochengdepianduanqueshihuoyimacuowu，shikangtikugoujianguochengzhongyidakunrao，erwosidejiegouneixuanzejishuyouxiaodejiejuelezheiyiwenti。