烟草（Nicotiana tabacum）shiyinianshengqiekecaobenzhiwu，qiyuanyuredaiheyaredaimeizhou，shishengwujiannigudingdefengfulaiyuan，zhangqizuoweicaoyaoheshehuixingfenjishiyong。daomuqianweizhi，yancaoyijingzaishijiefanweineizhongzhi，tabujinshizhuyaodejingjizuowu，erqieshiyanjiuzhiwuyichuanxue、fenzishengwuxuehexibaoshengwuxuedemoshizhiwu。
beijinganbiqishengwukejiyouxiangongsikeyitigongduozhongyancaoyichuanzhuanhuafangfa。muqianyingyongjiaoweiguangfandefangfashinongganjunjiedaodeyepanzhuanhuafa，gaifangfakeshixianyancaowendingzhuanhua，qiejuyouzhuanhuaxiaolvgaoheshiyanzhouqiduandeyoushi。tongshi，womenkeyishixianyancaoyepianbeimianzhushedeshunshizhuanhua（tu1），gaifangfakeyiyongyujiyindeshikongbiaodayanjiu。lingwai，haitigongjiyinqianghejuyierchunjiedaodeyuanshengzhitizhuanhua。
tu 1. nongganjunjiedaodeyancaoyepianweizhusheshunshizhuanhua（smith, 2012）。
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参考文献：
Smith R. H. 2012. Plant tissue culture Techniques and experiments. third edition. harcourt b. j., publishers. academic press, inc, san diego.
