北京安必奇生物科技有限公司拥有植物生物技术经验，可以提供水稻（Oryza sativa）yichuanzhuanhuafuwu。shuidaoshizhongyaodeguleizuowu，shijieshangyibanyishangderenkouyitaweizhushi。renmenyizhizhiliyushuidaopinzhihechanliangdetigao。ciwai，shuidaohaishiyanjiujiyingongnenghediaokongjizhidezhongyaomoshizhiwu。
nongganjunjiedaodeyichuanzhuanhuayouyuzhuanhuaxiaolvgao、t-dnazhenghekaobeishushao，yincimuqianshichangyongdeshuidaozhuanhuafangfa。womengongsiduozhongnongganjunjiedaodeshuidaozhuanhuafangfa，zhuyaoyouyixiajizhong：
gongpeiyangnongganjunyucongchengshuzhongzihuodedeyushangzuzhi（tu1）。
gongpeiyangnongganjunyubuchengshudepei。
zaiyongyetipeiyangjijinrunguodelvzhishanggongpeiyangshuidaoyushangzuzhihenongganjun。
gongpeiyangnongganjunyuxuanfuxibao。gaifangfakeyitigongzhuanhuaxiaolv。
jiangjinpaohoudechengshuzhongzidedingduanpeixingzuzhiyongzhenchuancihouyunongganjungongpeiyang。jiezhonghoudezhongzikebujingguozuzhipeiyangdeguochengzhijiezhangchengzhizhuranhoujiezi。
原位蘸花法。
beijinganbiqishengwukejiyouxiangongsiyouzhuanjia，bingqiewomenyuguoneiwaigaoxiaoheyanjiudanweiyouhezuo，keyigenjukehudexuqiutigongdingzhihuafuwu，zhuyaotixianzaiyixiajigefangmian：
zuzhipeiyanghezhiwushengzhangtiaojian。
多样的水稻品种： japonica 品种： Nipponbare、 Zhonghua11、 Akdong、 Suweon330、Anjungbyeo、 Taipei309、 Cheniere和Katy； indica pinzhong：9311、 minghui86、 minghui63、kasalath、asd16、white ponni、br29heir64。
nongganjunjunzhu：gv3101、agl-1、 eha101、 ;eha105hec58c1deng。
duozhongshaixuanbiaoji：kanamycin、hygromycin、phosphinothricinheg418deng。
womenqidaiyunindehezuo，ruyoujishuwentihuoxunjiakesuishilianxiwomendegongzuorenyuan，womenhengaoxingweininjieda！
图 1. 农杆菌介导的indicashuidaochengshuzhongziyoudaodeyushangzuzhizhuanhua（sahoo and tuteja, 2012）。
参考文献：
Sahoo R. K.; Tuteja N. (2012) Development of Agrobacterium-mediated transformation technology for mature seed-derived callus tissues of indica rice cultivar IR64, GM Crops and Food: Biotechnology in Agriculture and the Food Chain, 3 (2), 123-128.
