jianhuajiajihuacexu(rrbs, reduced representation bisulfite sequencing)shiyizhongzhunque、kuaisu、gaoxiao、jingjidednajiajihuayanjiufangfa,tongguoliyongxianzhixingneiqiemeiduijiyinzujinxingmeiqie,fujiqidongzijicpgdaoquyu,bingjinxingbisulfitechulijifujijianku,jieheilluminagaotongliangpingtaicexu,shiyizhonggaoxingjiabicexufangfa。
rrbs zuoweiyizhongguanzhucpg-rich quyudejiajihuayanjiufangfa,tongshishixiandnajiajihuazhuangtaijiancedegaofenbianlvhecexushujudegaoliyonglv,nenggoubimianguoduodeshujulangfei,gengyouzhenduixingdeyanjiufugaiquyudejiajihuazhuangtai,shidedaguimoyangbenyanjiuchengweikeneng,beiguangfanyingyongyudayangbendanjianjifenbianlvjiajihuachayifenxihejibingfenzibiaojikaifa。
我们的优势
danjianjifenbianlv,zuodaojiajihuaweidiandedingxinghexiangduidingliangfenxi
成熟的建库技术
duoyangbendefugaiquyuzhongfuxinggao
稳定高效的项目流程
zhouqiduan,youjingzhenglidejiageyoushi
技术流程
数据分析
标准分析
原始数据质控
比对到参考基因组
甲基化位点识别
promoterhecpgdaofugaifenxi
promoterhecgiqudejiajihuafenxi
duoyangbenjiajihuachayifenxi:juleifenxi;gofenxi;keggfenxi
高级分析
chayibiaodajiyinqidongziqudejiajihuashuipingfenbu
jiajihuaxiushiyubiaodadiaokongguanxi
样本要求
样本类型 | 总量 | 浓度 | OD260/OD280 |
基因组DNA | ≥1 μg | ≥20 ng/μl | ≥ 1.8 |
技术参数
测序平台 | 文库类型 | 测序深度 | 项目周期 |
Illumina 150PE | 350 bp | 30 M reads/1G基因组大小 | 视具体项目情况而定 |