rna immunoprecipitation (rip) shiyanjiuxibaoneirnayudanbaijieheqingkuangdejishu,shilejiezhuanluhoudiaokongwangluodongtaiguochengdeyouligongju,nengbangzhuwomenfaxianmirnadediaojiebadian。zheizhongjishuyunyongzhenduimubiaodanbaidekangtibaxiangyingderna-danbaifuhewuchendianxialai,ranhoujingguofenlichunhuajiukeyiduijiehezaifuhewushangdernajinxingcexufenxi。
anbiqideshengwuxinxituanduiweinintigongyizhanshiderip-seqjishufuwu,congcailiaoxuanqu,jiankucexudaoshujufenxi,meiyibudoujingguokexuezhenmidesheji,weininshendujieximubiaornazhongleiyijiqiyudanbaidexianghuzuoyong,baozhanggaozhiliangdeyanjiuchengguo。
我们的优势
xingjiabigao:shiyanzhouqiduan、fenxixinxiquan,jiagedi
gaofugaidu:quanzhuanluzudefugai,kezaiquanzhuanluzufanweineiduidanbaijieheweidianjinxingshaixuanhejianding
gaolingmindu:meigeyangbenkehuodeshubaiwantiaodexuliebiaoqian,kefaxianyanjiuzhuanluzushangxiyoudedanbaijieheweidian
gaojingquelv:kehuodegaoshuipingdexinzaobishuju,zhunquequfenzhenshishijianyuzaoyin,jingquedingweidanbaijieheweidian
dingzhihuadernamianyigongchendiancexufangan:kegenjushijixuqiuzhidinggexinghuadernamianyigongchendiancexufangan
技术流程
caiyongteyixingkangtiduimudedanbaijinxingmianyichendianhou,fenliyuqijiehedernapianduan,zaitongguogaotongliangcexuyushujufenxi,zaiquanzhuanluzufanweineishaixuanjijiandingmudedanbaidernajieheweidian,bingqiekeyijiyuduogeyangpinjinxingchayibijiao。
数据分析
样本要求
样本类型 | 总量 | 浓度 | OD260/OD280 |
DNA | ≥50ng | ≥30ng/μl | 1.8-2.0 |
技术参数
测序平台 | 测序策略 | 数据量 | 运转周期 |
HiSeq | SE 50 | 20M clean reads | 根据具体项目情况而定 |
询价及订购
ganxieninxuanzeanbiqirip-seqcexufuwu。ruyourenhexiangguandewenti,qingninlianxiwomendegongzuorenyuan,womenjikeweininjiedahefuwu。
参考文献
bersani c, huss m, giacomello s, et al. genome-wide identification of wig-1 mrna targets by rip-seq analysis [j]. oncotarget, 2016, 7(2): 1895.
liu n, dai q, zheng g, et al. n6-methyladenosine-dependent rna structural switches regulate rna-protein interactions [j]. nature, 2015, 518(7540): 560-564.
kanematsu s, tanimoto k, suzuki y, et al. screening for possible mirna–mrna associations in a colon cancer cell line [j]. gene, 2014, 533(2): 520-531.