small rnashishengminghuodongzhongyaodediaokongyinzi，jihucunzaiyusuoyoudeshengwutizhong，zhuyaobaokuomirna、pirna、sirnahencrnadeng。small rnacanyuxuduozhongyaodeshengwuxueguocheng，zaijiyinbiaoda、shengwugetifayu、daixiejijibingdefashengdengguochengzhongqizhezhongyaodezuoyong。
small rnacexushiyanjiushengwuyangpinxiaornadezhuyaofangfazhiyi，shouxiancaiyongjiaofenlijishu，shoujiyangpinzhong18-30ntdernapianduan，zaitongguogaotongliangcexujishu，duiyangbenzhongsuoyousmall rnajinxingcexuhebiaodadingliang，yicixinghuodedanjianjifenbianlvdeshubaiwantiaosmall rnaxuliexinxi。
anbiqismall rnacexujishufuwujuyoupingtaiyou、tonglianggao、xingjiabigaodengyoudian，zhizaiweinindesmall rnacexuxiangmutigongwanzhengdejiejuefangan。congcailiaoxuanqu，jiankucexudaohouxudeshujufenxi，douyoubiaozhunhuadecaozuoliucheng，baozhengyanjiuchengguodegaozhiliang。
我们的优势
gaofenbianlv、gaojingquedu：danjianjichayidejiance，congjigedaoshuwangekaobeijingquejishu
gaotongliang、gaozhiliang：yicicexudedao800wantiaoyishangdexulie，shiyanzhouqiduan、xiaolvgao、chengbendi、zhiliangkekao
biaozhunhuadefenxiliucheng：zhuanyedeshengwuxinxituanduiyijifenxifuwutixi，weinintigongbiaozhunfenxi、gaojifenxiyijidingzhihuafenxi
fengfudexiaornacexujifenxijingyan：zhuanyedecexujishurenyuanxiezhushejishiyanfangan、jiejueshiyanwentibingfenxishiyanjieguo
dingzhihuadexiaornacexufangan：kegenjushijixuqiuzhidinggexinghuadexiaornacexufangan
技术流程
数据分析
样本要求
| 样本类型 | 总量 | 浓度 | OD260/OD280 |
| RNA | ≥3 μg | ≥200ng/μl | 1.8-2.2 |
| 血清血浆 | 20ng | ||
| FFPE | 200ng |
技术参数
| 测序平台 | 测序策略 | 指标 | 运转周期 |
| HiSeq 2500 | SE50 | 一般深度：6-10M clean reads; 高深度：15-20M clean reads | 45个工作日 |
询价及订购
ganxieninxuanzeanbismall rnacexufuwu。ruyourenhexiangguandewenti，qingninlianxiwomendegongzuorenyuan，womenjiechengweininjiedahefuwu。
参考文献
joshi t, elias d, stenvang j, et al. integrative analysis of mirna and gene expression reveals regulatory networks in tamoxifen-resistant breast cancer [j]. oncotarget, 2016, 7(35): 57239-57253.
gao s, yang l, zeng h q, et al. a cotton mirna is involved in regulation of plant response to salt stress [j]. scientific reports, 2016, 6.
li a, liu d, wu j, et al. mrna and small rna transcriptomes reveal insights into dynamic homoeolog regulation of allopolyploid heterosis in nascent hexaploid wheat [j]. the plant cell, 2014, 26(5): 1878-1900.
