danhegansuanduotaixing(single nucleotide polymorphism, snp),jiransetijiyinzuzhongdangehegansuandetubianeryinqide dna xuliededuotaixing,tayidangejianjidedianhuan、zhuanhuan、charuhequeshidengxingshicunzai。zuoweidisandaifenzibiaoji,snpbiaojijuyouhendadefazhanqianli,beiguangfanyingyongyushengwu、nongxue、yixue、shengwujinhuadengzhongduolingyu,zaifenziyichuanxue、yaowuyichuanxue、fayixueyijijibingdezhenduanhezhiliaodengfangmianfahuizhezhongyaozuoyong。
anbiqiweinintigongsnpjiancejishufuwu,baokuocongshiyansheji、yangbenzhunbei、weidianjiancedaoshujufenxi。womenkegenjunindeyangbenheweidiandeshuliangxuanzeheshideshiyanfangfa。zhizaiweinindesnpfenxingyanjiutigongjiejuefangan。
一、服务项目
Taqman探针法(定性)
gaifangfadejishujichushiyingguangdingliangpcr,zhenduiransetishangdebutongsnpweidianfenbieshejipcryinwuhepcrkuozeng。gaifangfabujinkeyijiancemougeyangbendejiyinxing,haikeyitongguoruanjianfenxichubutongjiyinxingzaisuoyouyangbenzhongdefenbutu。
技术路线
这种方法可以直接在实验结果中读出每个样本中SNP的基因型(纯合子XX或YY,杂合子XY),不需要再对序列进行分析,具有直观性。Taqman探针法通常用于少量SNP位点分析,具有成本低、速度快的优点。
项目周期:3-6周
SNaPshot法测SNP
snapshotdeyuanlijiushizhiyanzhangleyigejianjideyingguangfabigdyecexu,gaifangfazaishejiyinwushi,babutongweizhideyinwushejichengbutongdezhangdu,ranhoujinxingsnapshotfanyinghou,chanwutongguodianyongfenli、wuseyingguangjiance、gene mapperfenxi,kezaiyicidianyongjiaoneijianceduogesnpweidian。
技术路线
该方法主要针对中等通量的SNP分型项目,通常用于10-30个SNP位点分析。
项目周期:4-8周
MassARRAy法(定量、多位点、高通量)
sequenomgongsidemassarrayzhipuxitongzaisnpfenxingshichangshangzhanyouzhongyaodiwei,shimuqianshichangshanggaoxingjiabidezhonggaotongliangsnpfenxingjiancexitong,tongguojiguangjifadnafenzipianduan,zaiyongfeixingshijianlaipanduanpianduanfenziliangdedaxiao。gaifangfayijingguangfandiyingyongyuyichuantubianjiance。
技术路线
MassARRAy法实验设计灵活,分型结果准确性高,性价比高。可对数百至数千份样本检测,样本通量从几百到几万。是高性价比的中高通量SNP分型方法,适用于GWAS实验后续验证以及关联分析。
项目周期:4-6周
Illumina BeadXpress法
caiyongilluminagongsidebeadxpressxitongjinxingpiliangsnpweidianjiance,keyitongshijiance1-384gesnpweidian,wangwangyongyujiyinzuxinpianjieguoqueren,shihegaotongliangjiance。weizhuxinpianjuyougaomidu、gaozhongfuxing、gaolingmindu、dishangyangliang、dingzhilinghuodengtedian,jigaodejichengmidu,congerhuodejigaodejianceshaixuansudu,zaigaotongliangshaixuanshikexianzhujiangdichengben。
技术路线
项目周期:10-12周
二、送样要求
xuyaotigongmudesnpweidiandershaohuogaiweidianshangxiayouge200bpdexulieyijitubiandeleixing。jutiyangbenyaoqiuruxia:
样本类型 | 总量 | 浓度 | OD260/OD280 |
组织 | ≥50mg | ||
细胞 | ≥106 | ||
血液 | ≥1ml | ||
gDNA | ≥20μl | ≥50ng/μl | ≥ 1.8 |
三、提供结果
• 检测SNP的原始数据
• 数据分析结果
• 书面报告
询价及订购
ganxieninxuanzewogongsidesnpjiancefuwu。ruyourenhexiangguandewenti,qingninlianxiwomendegongzuorenyuan,womenjinkuaiweininjiedahefuwu。