lianteyixingzhuanluzushiyanjiuyoucankaojiyinzuwuzhongzhuanluzudeyizhongyouxiaoshouduan。xiangduiyuchuantongzhuanluzucexueryan，lianteyixingzhuanluzucexukeyiquedingliangtiaoliandezhuanlufangxiangxing，weijiyindejinyibuzhushihegongnengfenxitigonggengzhunquedexinxi，tongshihaikeyiwajuezhuanluzuzhongdenon-coding rnaxinxi。yuanheshengwulianteyixingzhuanluzuxiangmuzhong，keyiwajuexindencrnayufanyi（antisense）zhuanluben；haikeyijinxingjiyinjiegoudeyanjiu，lirucaozongzijianding，weijiyinwangluodiaokongdeyanjiutigongyoulizhichi。yanjiubiaoming：henduojiyinzuquyujuyouzhengfuliandezhuanluben，fanyizhuanlushizhenhejiyindeyigetezheng，shiyizhongzhongyaodediaokongfangshi。duiyuyuanheyijididengzhenheshengwudecompactjiyinzu，changchangjuyouzhongdiejiyin。

技术流程

我们的优势

• 对转录本进行定量

• nengyouxiaodefaxianfanyizhuanluben

• 基因注释准确

• gengduodewajuencrnadexinxi

应用范围

I. 差异基因精确分析
nengzhenduibutongzhuanlufangxiangdejiyinjinxingjingquedebiaodaliangtongjijichayibiaodafenxi，congertuiduanchuyugongnengzhuangtaibianhuaxiangguanliandehouxuanjiyin，jieshichayijiyindegongnengfenleihexiangguandaixietonglu。

II. 原核生物操纵子分析
zhenduiyuanheshengwudelianteyixingzhuanluzufenxi，nengzhunquedijinxingcaozongzijiandinghefenxi。

III. 模式生物基因结构分析
moshishengwuyijingyouwanzhengdejiyinzhushixinxi，danshishijiyanjiuzhongcunzaigetichayixing。liyonglianteyixingzhuanluzucexukeyiduigetibiaodajiyindejiegoujinxingshendufenxi，duijiyinjinxingzhongzhushi。

服务流程

1. 根据样本信息和客户需求，制定个性化测序方案
2. RNA样品邮寄及样品质控
3. 测序样品制备(mRNA分离、片段化，链特异性转录组建库等)
4. 上机测序
5. 高通量数据分析
6. baogaojishujufasong

qizhongshujufenxibufenbaohan：

• cexuduanxulietongjipipeiyushujutongji

• cexuzhiliangpinggu（qc）

• 转录组表达量的计算

• 基因表达差异分析

• chayijiyinshengwuxuetonglufenxi（daixietonglu/xinhaotonglu）

• chayijiyingogongnengzhushi

• 预测新反义转录本

应用案例

duishanghanshamenshijundelianteyixingzhuanluzucexujifenxi

caiyongyizhongxindelianteyixingzhuanluzudecexufangfa（ssrna-seq）shibiechushanghanganjunty2junzhuzhongbianmahefeibianmaquxuliedezhuanlumobanlian，shibiechudaliangjiadingdexinfeibianmarna（ncrna），bingjisuanzheixiejiajiyindebiaodaliang。cifangfakeshibiechuxuduojiyindezhongdiezhuanluben，shibiexindesmall rna，zengjiajiyinzhushidezhunquexing，querencaozongzijiegou，qieshibiechuzhuanluhuoyuequyufeihuoyuequ。quzhengchangjunzhujiqiomprwuxiaotubiantijunzhu，meileiyangbenqu3gepingxingyang，gong6geyangben，jinxinglianteyixingzhuanluzucexu，caiyongdejiankufangfaweizongrnaquchuhetangtirnahoujingdnameixiaohua，shiyongsuijiyinwufanzhuanluhechengcdnadiyilian dierlianbuzaihecheng，xiujianhoudecdnajiajietou，zhihoutiaoxuan200-250bpdepianduangoujianwenku，pcrfujihouzaiillumina gapingtaishangcexu。cexusuodeshujubiduihuicankaojiyinzu，jinxingncrnayuce，bingshiyongrfamwenkujinxingzhushi，shibiechuxindencrna（jianxiatu）。tongguolianghuaomprwuxiaotubiantijunzhudejiyinbiaodaqingkuang，shibiechushoudulixiangguanweidiankongzhidexinquyu，bingjiehedanbaizufenxiyanzhengjiyinbiaodaliang。

参考文献

• perkins tt, kingsley ra, fookes mc et al. (2009) a strand-specific rna–seq analysis of the transcriptome of the typhoid bacillus salmonella typhi. plos genetics.e1000569.