absolute mag™ tev proteasecizhushiyizhongbiaomiangudingyoutev protease (tevp)decixingkeli，zhuyaoyongyuronghedanbaishangdebiaoqiandequchu，shiyonghoudedanbaimeicizhukeyihuishouzhongfuliyong。tevpshiyouyancaoshikebingdujiyinzuzhongdenla（hebaohandanbaijiyin）kongzhibiaoda，fenziliangyuewei28 kda，keyishibieglu-asn-leu-tyr-phe-gln-glyqianjisuanxulie，bingzaiguanxianan（gln）heganansuan（gly）zhijianjinxingqiege，ruguobiaoqianhemudedanbaizhijianshitongguogaiqianjisuanxuliejinxingronghe，zekeyibeitevpyouxiaoqiege。tevpjuyougaoduteyixinghelianghaodecuihuahuoxing，shimuqianguangfanyongyuronghedanbaibiaoqianquchudedanbaimei。

absolute mag™ tev proteasecizhu，liyongtevphelianmeiqinhesuronghexingchengyizhongfuhedanbai，ranhouyubiaomianshengwusubaobeidecizhuxianghunhe，tongguolianmeiqinhesuheshengwusudeqinhezuoyong，congershitevpgudingdaocizhubiaomian，xingchengtevpcizhu。gaicizhubujinkeyiyouxiaoquchubiaoqian，erqiefanyinghoukeyizhijieshiyongcitiexifujiangdanbaimeibiaojicizhuconghunhedanbairongyezhongxunsufenli，erwuxuxiangchuantongdezhongzudanbaimeibixujingzhuchunhuacainengquchu，jidadijianhualedanbaichunhuadebuzhou，beixiaohuahoudemudedanbaihebiaoqianhunhewuzhixujingguobiaoqianxifuzhuquchubiaoqianjikededaochunhuademudedanbai。

产品特点：

• zhijietongguotevpcizhuchunhuadanbai，buxuyaozhucengxicaozuo；

• jianshaoxiayoudanbaimeiwuran；

• keyizhongfuliyongyigeyue；

• shiyongyuqinhebiaoqiandeqiege，danbaizhizhongtevweidiandejianding，yijigongchenghuadanbaizhidehuohua/shihuo。

使用方法：

（1）向管中加入磁珠，Buffer和目标蛋白，充分混匀；
（2）30℃下，在末端旋转器上孵育6小时或过夜；
（3）孵育过后，将溶液置于磁铁上吸附5分钟后，分离出的上清液即为切割后的融合蛋白和标签的混合物；
（4）jianghunhewuanzhaoxuyaojinxingchuli；cizhukeyiyongstorage buffer (pbs buffer) qingxi3x5 minhou，cunfangzai4℃zaiciliyong。

建议使用：

（1）每250 μl-1 ml反应体系，建议使用50-100 μl磁珠悬浮液。如果磁珠保存在4℃条件下，可以维持活性超过1个月。磁珠可以重复用于多种蛋白酶反应。
（2）xiangfanyingtixizhongtianjiazhongnongduwei10 mmdedtthuoβ-me，keyihuodejiaojiadediwudanbaishuijiexiaoguo。

实验数据：

yimbp-enlyfqs-gfpdiwudanbaibiaoqianquchuweili。zuotuweiabsolute mag™ tev proteasecizhuzai4℃he30℃xialiejiembp-enlyfqs-gfpdiwudanbaidesds-pagefenxi。zai30℃xia，200 μlde74.6 kdadiwudanbai（0.1 mg/ml）yu50 μl tevpcizhufuyuguoyehou，beiyouxiaoliejie90%。erzai4℃xiaguoyefuyu，qiegexiaolvjiangdiwei21%。youtuweiabsolute mag™ tev proteasecizhuzai4°cxiachucunyigeyuehoujinxingmeihuojiance。sds-pageningjiaoxianshizai30℃xia200 ulde74.6 kdadiwudanbai（0.1mg / ml）yu50 ul tevpcizhufuyuguoyehou，yiranbeiyouxiaoqiege（90%）。