小麦（Triticum aestivum）shishijieshangzhongyaodenongzuowuzhiyi。xiaomaijuyoujiaogaodeyingyangjiazhihejingjijiazhi，yeshiyanjiuzhiwushengzhangdiaokonghezuowuzengchandezhongyaoguleimoshishengwu。
suizheshichangxuqiuderiyizengzhang，xuyaopeiyugaochan、kangnixingqiangdexiaomaipinzhong。yinci，jiyingongchengjishuzaixiaomaizhongdeyingyongyichengweixiandainongyeyanjiuderedian。zaixiaomaiyichuanzhuanhuazhong，yingyongguangfandezhuanhuafangfashijiyinqianghenongganjunjiedaodezhuanhua。zheiliangzhongzhuanhuafangfadoukeyizhuanhuayouduozhongwaizhitihuodedeyushangzuzhi，ruxinxianfenlideweichengshupeitai、yupeiyangdeweichengshupeitai，huocongchengshuhuoweichengshupeitaizhongtiqudepeixingyushangzuzhi。zhuanhuahoudeyushangzuzhikejinyibupeiyangzaishengweikeyudezhuanjiyinxiaomaizhizhu（tu1）。lingwai，womenyeketigongbujingzuzhipeiyangguochengdeyuanweixiaomaizhuanhuafangfa，zheixiefangfazhuyaobaokuoyixia3zhong：
jiyinqianghongjiganxizhangzhongzidedingduanfenshengzuzhi。
jiangjinpaohoudechengshuzhongzidepeidingduanfenshengzuzhiyongzhenjinxingchuanci，ranhouyunongganjungongpeiyang。
jiangxiaomaidesuizhuanghuaxuzaiyunsuiqiyongnongganjunjinxingshentouchuli。
tu 1. yunyongchengshupeijinxingxiaomaiyichuanzhuanhuaguocheng（medvecká and harwood, 2015）。
beijinganbiqishengwukejiyouxiangongsikeyiweikehutigongxiaomaiyichuanzhuanhuafuwu，baokuoshiyansheji、zaitigoujian、zhilizhuanhua、yangxingyizhishaixuanhejiancedenghuanjie。womenketigongduogexiaomaipinzhongdezhuanhua：rukenong199、 longchun23、bobwhite、 fielder、 westonia、gascoigne、 sunvale、sunstate、stewart、 gladiushejanzdeng。haiketigongduogenongganjunjunzhu：ru gv3101、agl-1、 eha101、 eha105hec58c1heduozhongshaixuanbiaoji：rukanamycin、 hygromycin、 phosphinothricinhe g418deng。 womenjuyouduoniandejingyanhezhishi，keyibangninjiejuexiaomaizhuanhuazhongdejishuwentihetiaozhan，yizhulininxiangmudechenggong。
ninyourenhexiangguanwentikelianxiwomendegongzuorenyuan，womensuishiweininjieda！
参考文献：
Medvecká, E.; Harwood, W. A. (2015). Chapter 16 Wheat (Triticum aestivum L.) transformation using mature embryos. Methods in Molecular Biology, 1223, 199-209.
