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安必奇的生物信息团队为您提供一站式的基因组de novo测序技术服务,包括从实验设计、样本准备、上机测序到生物信息学分析,旨在为您的基因组学提供解决方案。我们的基因组从头测序技术服务包括:
1. 动植物基因组de novo测序
2. 细菌基因组de novo测序
3. 真菌基因组de novo测序
4. hongjiyinzude novocexu
我们的优势
pingtaiyoushi:huoqugaozhiliangdejiyinzutupu
gaoshenducexu:zhunquelvgao
fengfudeshujufenxineirong:biaozhunfenxi、gaojifenxi、dingzhihuafenxi
fengfudejiyinzucexujifenxijingyan:yiwanchengduoxiangdaxingjiyinzucexuxiangmu
dingzhihuadejiyinzucexufangan:kegenjushijixuqiuzhidinggexinghuadejiyinzucexufangan
技术流程
数据分析
i. dongzhiwujiyinzude novocexushujufenxi
ii. xijunzhenjunjiyinzude novocexushujufenxi
提供结果
biduihecountsshujisuan
差异基因表达分析
差异转录本表达分析
发现新型异构体
zuzhuangde novozhuanluzu
分析基因GO功能
定制化信息分析
样本要求
样本类型 | 总量 | 浓度 | OD260/OD280 |
动物组织 | >500mg | ||
动物组织 | >500mg | ||
DNA | >400μg | ≥30ng/μl | ≥ 1.8 |
技术参数
项目类型 | 测序平台 | 测序策略 | 数据量 | 运转周期 |
动植物基因组de novo测序 | HiSeq | PE150 | Contig N50 ≥ 20 kb Scaffold N50 ≥ 300 kb | 视具体项目情况而定 |
细菌基因组de novo测序 | HiSeq | PE150 | ≤30 scaffolds | 视具体项目情况而定 |
真菌基因组de novo测序 | HiSeq | PE150 | Scaffold N50≥300kb | 视具体项目情况而定 |
询价及订购
ganxieninxuanzeanbiqidequanjiyinzude novocexufuwu。ruyourenhexiangguandewenti,qingninlianxiwomendegongzuorenyuan,womenjikeweininjiedahefuwu。
参考文献
li, ruiqiang, et al. "de novo assembly of human genomes with massively parallel short read sequencing." genome research 20.2 (2010): 265-272.
huang j, zhao y, shiraigol w, et al. analysis of horse genomes provides insight into the diversification and adaptive evolution of karyotype [j]. scientific reports, 2014, 4.