玉米（Zea mays）bujinshiyanjiuzhiwuyichuanxue、jiyinzuxuehefenzishengwuxuedezhongyaomoshizhiwu，haishijuyoujiaogaojingjijiazhidezhuyaoguleizuowu。suizhezhuanjiyinjishuzaixiandainongyezhongdefazhanheyingyong，renmenyijingpeiyuchulejuyoukangchucaojihe/huokangchong、naihanhuogaotanshuihuahewuhanliangdezhuanjiyinyumichanpin。
nongganjunjiedaodeyichuanzhuanhuashiyumizhuanjiyinchangyongdefangfa。gaifangfadejianyaobuzhouruxia：shouxian，fenliweichengshudeyumipeijiangqiyuxiedaiyoumudejiyinzaitidenongganjungongpeiyang；qici，youdaozhuanhuahoudeweichengshupeichanshengyushangzuzhi；ranhou，yushangzuzhizaishengbingjinxingyangxingmiaoshaixuan（tu1）。nongganjunjiedaodezhuanhuafangfakeyichanshenghanyoudangehuoxiangduijiaodizhuanjiyinkaobeishudezhuanjiyinyumigeti。nongganjunjiedaodezhuanhuafangfachuleyongyuweichengshudepeiwai，haikeyiyingyongyuqitazuzhi，ruxinqiegedejingjianhuozhitiao。duozhongwaizhitidexuanzekeyishinongganjunjiedaodezhuanhuafangfashiyongyuduozhongyumijiyinxing。
图1. 农杆菌介导的玉米遗传转化（Zhou et al., 2011）。
beijinganbiqishengwukeweinintigongyumiyichuanzhuanhuafuwu。womenkeyigenjunindexuqiutigongdingzhihuadeyumizhuanjiyinfuwu。womenyouduozhongyumijiyinxing、nongganjunzhu、kelongzaitiyijipeiyangjipeifanggongninxuanze：
玉米基因型：zijiaoxi： a188、 a634、 h99、 w117、 y423、 phr03、 mo17、b104、b114he ky21；zajiaoxi： hi-ii。
农杆菌株：gv3101、agl-1、eha105、lab4404hec58c1。
载体：shuangyuanzaitihejuyouzhuanhuaxiaolvdechaoshuangyuanhuosanyuanzaitixitong。
培养基配方：peiyangji、shiyongyuhi-iidediyanpeiyangji（peihekangyanghuajil-banguangansuanhehuodttshiyong）、shiyongyuphr03degongpeiyanghexuanzepeiyangdehangaotonghe6-bianjianjipiaolingdepeiyangjideng。
ruyourenhewenti，qinglianxiwomendegongzuorenyuan，womenhengaoxingweininfuwu！
参考文献：
Zhou, R.; et al. (2011). Transformation of, GBSS I, gene driven with barley endosperm-specific promoter into maize inbred line mediated by Agrobacterium. Maize Genomics & Genetics, 3(1), 1-5.
